EMDB EMD-6777

Single particle reconstruction
3.2Å resolution

Electron cryo-microscopy structure of LbuCas13a-crRNA binary complex

Map released:
Overview of EMD-6777
Source organism: Leptotrichia buccalis [523794]
Fitted atomic model: 5xwy
Primary publication:
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.
Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y
Cell 170 714-726.e10 (2017)
PMID: 28757251

Function and Biology Details

Sample name: LbuCas13a-crRNA binary complex
Nucleic acid: RNA (59-MER)
Proteins: LbuCas13a-crRNA binary complex, A type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, Cas13a

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)