EMD-6772

Single-particle
3.7 Å
EMD-6772
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Deposition: 18/06/2017
Map released: 30/08/2017
Last modified: 06/09/2017
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6772
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Cryo-EM structure of human respiratory complex I transmembrane arm

EMD-6772

Single-particle
3.7 Å
EMD-6772
3D View Gallery
Deposition: 18/06/2017
Map released: 30/08/2017
Last modified: 06/09/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.2 mg/mL
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 1.25 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 167761
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 1.4 -
⦨ Initial angle assignment
Type: RANDOM ASSIGNMENT
Software [1]
Name Version Details
RELION 1.4 -
⦩ Final angle assignment
Type: RANDOM ASSIGNMENT
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Software [1]
Name Version Details
CTFFIND 3.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This map was obtained by sub-region refinement.
⬡ Geometry
X Y Z
Dimensions 480 480 480
Origin 0 0 0
Spacing 480 480 480
Voxel size 1.083 Å 1.083 Å 1.083 Å
Contour list
Primary Level Source
True 0.0677 AUTHOR