EMDB EMD-6721

Single particle reconstruction
3.6Å resolution

Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom

Map released:
Overview of EMD-6721
Source organism: Homo sapiens [9606]
Fitted atomic model: 5xjc
Primary publication:
An Atomic Structure of the Human Spliceosome
Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y
Cell 169 918-929.e14 (2017)
PMID: 28502770

Function and Biology Details

Sample name: human C* spliceosome
Ligands: INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ZINC ION, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: U5 snRNA, U6 snRNA, pre-mRNA, Homo sapiens small nuclear RNA (U2) gene
Proteins: human C* spliceosome, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 small nuclear ribonucleoprotein 40 kDa protein, Pre-mRNA-splicing factor SYF1, Crooked neck-like protein 1, Pre-mRNA-splicing factor SPF27, Cell division cycle 5-like protein, Pre-mRNA-splicing factor SYF2, Protein BUD31 homolog, Pre-mRNA-splicing factor RBM22, Spliceosome-associated protein CWC15 homolog, Intron-binding protein aquarius, SNW domain-containing protein 1, Peptidyl-prolyl cis-trans isomerase-like 1, Pleiotropic regulator 1, Serine/arginine repetitive matrix protein 2, Pre-mRNA-splicing factor CWC22 homolog, Pre-mRNA-processing factor 17, PRKR-interacting protein 1, ATP-dependent RNA helicase DHX8, Pre-mRNA-splicing factor SLU7, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', Pre-mRNA-processing factor 19, Eukaryotic initiation factor 4A-III, Protein mago nashi homolog 2, RNA-binding protein 8A, Protein CASC3

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)