EMDB EMD-6709

Single particle reconstruction
3.4Å resolution

Structure of the 70S chloroplast ribosome from spinach

Map released:
Last modified:
Overview of EMD-6709
Source organism: Spinacia oleracea [3562]
Fitted atomic model: 5x8p
Related EM entry by publication: EMD-6710, EMD-6711
Primary publication:
Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation
Ahmed T, Shi J, Bhushan S
Nucleic Acids Res. 45 8581-8595 (2017)
PMID: 28582576

Function and Biology Details

Sample name: Chloroplast 70S ribosome from spinach
Nucleic acids: 5S rRNA, 4.8S rRNA, 23S rRNA, 16S rRNA
Proteins: Chloroplast 70S ribosome from spinach, chloroplast ribosome 50S large subunit, chloroplast ribosome 30S small subunit, 50S ribosomal protein L32, chloroplastic, 50S ribosomal protein L33, chloroplastic, 50S ribosomal protein L34, chloroplastic, 50S ribosomal protein L35, chloroplastic, 50S ribosomal protein L36, chloroplastic, protein cL37, protein cL38, 50S ribosomal protein L2, chloroplastic, protein L3, 50S ribosomal protein L4, chloroplastic, 50S ribosomal protein L5, chloroplastic, protein L6, protein L9, 50S ribosomal protein L13, chloroplastic, 50S ribosomal protein L14, chloroplastic, protein L15, 50S ribosomal protein L16, chloroplastic, protein L17, protein L18, 50S ribosomal protein L19, chloroplastic, 50S ribosomal protein L20, chloroplastic, 50S ribosomal protein L21, chloroplastic, 50S ribosomal protein L22, chloroplastic, 50S ribosomal protein L23, chloroplastic, 50S ribosomal protein L24, chloroplastic, protein L27, protein L28, protein L29, 50S ribosomal protein L31, 30S ribosomal protein S2, chloroplastic, 30S ribosomal protein S3, chloroplastic, 30S ribosomal protein S5, chloroplastic, 30S ribosomal protein S6 alpha, chloroplastic, 30S ribosomal protein S7, chloroplastic, 30S ribosomal protein S8, chloroplastic, 30S ribosomal protein S9, chloroplastic, protein S10, 30S ribosomal protein S11, chloroplastic, 30S ribosomal protein S12, chloroplastic, 30S ribosomal protein S13, chloroplastic, 30S ribosomal protein S15, chloroplastic, 30S ribosomal protein S16, chloroplastic, protein S17, 30S ribosomal protein S18, chloroplastic, 30S ribosomal protein S19 alpha, chloroplastic, protein S20, protein S21, protein plastid pY, protein cS23, 30S ribosomal protein S4, chloroplastic, protein cS22, 30S ribosomal protein S14, chloroplastic, protein bTHXc, 30S ribosomal protein S1, chloroplastic

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: Relion
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)