EMDB EMD-6209

Single particle reconstruction
8.2Å resolution

Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIb

Map released:
Last modified:
Overview of EMD-6209
Source organisms:
Fitted atomic model: 3j99
Related EM entries by publication: EMD-6204, EMD-6205, EMD-6206, EMD-6207, EMD-6208, EMD-6210
Primary publication:
Mechanistic insights into the recycling machine of the SNARE complex
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
NATURE 518 61-67 (2015)
PMID: 25581794

Function and Biology Details

Sample name: 20S supercomplex consisting of truncated neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Proteins: N-ethylmaleimide sensitive factor, alpha Soluble NSF Attachment Protein, Syntaxin-1A, Synaptobrevin-2, Synaptosomal-associated protein 25

Experimental Information Details

Resolution: 8.2Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 (4k x 4k)