EMD-6207

Single-particle
7.8 Å
EMD-6207
3D View Gallery
Deposition: 05/12/2014
Map released: 28/01/2015
Last modified: 11/02/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6207
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j97 summary report (pdf.gz) Map and 3j97 FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state II

EMD-6207

Single-particle
7.8 Å
EMD-6207
3D View Gallery
Deposition: 05/12/2014
Map released: 28/01/2015
Last modified: 11/02/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: 20S supercomplex consisting of truncated neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Sample [1]
Name: 20S supercomplex consisting of truncated neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Number of unique components: 5
Oligomeric state: One hexamer of NSF + four alpha-SNAP molecules + one SNARE complex
Molecular weight
Experimental Theoretical Method
- 660 kDa -
Protein [5]
Recombinant expression
Name: N-ethylmaleimide sensitive factor (NSF)
Number of copies: 6
Oligomeric state: hexamer
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Cricetulus griseus (Chinese hamster)
Molecular weight
Experimental Theoretical Method
- 83 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21-DE3-RIL - pROEX-1
Recombinant expression
Name: alpha Soluble NSF Attachment Protein (alpha-SNAP)
Number of copies: 4
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Rattus norvegicus (Rat)
Molecular weight
Experimental Theoretical Method
- 33 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pJ414
Recombinant expression
Name: Syntaxin-1A (Stx-1A)
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Rattus norvegicus (Rat)
Molecular weight
Experimental Theoretical Method
- 8 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pACYC-DUET-1
Recombinant expression
Name: Synaptobrevin-2 (Syb-2, VAMP2)
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Rattus norvegicus (Rat)
Molecular weight
Experimental Theoretical Method
- 8 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pACYC-DUET-1
Recombinant expression
Name: Synaptosomal-associated protein 25 (SNAP-25)
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Rattus norvegicus (Rat)
Molecular weight
Experimental Theoretical Method
- 16 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pET-DUET-1