EMDB EMD-6165

Single particle reconstruction
13Å resolution

Essential Structural and Functional Roles of the Cmr4 Subunit in RNA Cleavage by the Cmr CRISPR-Cas Complex

Map released:
Last modified:
Overview of EMD-6165
Source organisms:
Related EM entry by publication: EMD-6166, EMD-6167
Primary publication:
Essential Structural and Functional Roles of the Cmr4 Subunit in RNA Cleavage by the Cmr CRISPR-Cas Complex
Ramia NF, Spilman M, Tang L, Shao Y, Elmore J, Hale C, Cocozaki A, Bhattacharya N, Terns RM, Terns MP, Li H, Stagg SM
Cell Reports 9 1610-1617 (2014)
PMID: 25482566

Function and Biology Details

Sample name: Cmr complex with crRNA and target RNA
Nucleic acids: crRNA, target RNA
Proteins: Cmr1, Cmr2, Cmr3, Cmr4, Cmr5, Cmr6

Experimental Information Details

Resolution: 13Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN1, Spider
Microscope: FEI TITAN KRIOS
Detector: GATAN ULTRASCAN 4000 (4k x 4k)