EMDB EMD-5860

Single particle reconstruction
21Å resolution

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Map released:
Last modified:
Overview of EMD-5860
Source organisms:
Related EM entry by publication: EMD-5858, EMD-5859
Primary publication:
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
SCIENCE 343 1247997 (2014)
PMID: 24505130

Function and Biology Details

Sample name: Cas9 loaded with crRNA:tracrRNA and bound to target DNA duplex
Nucleic acids: 55 bp DNA duplex target strand, CRISPR RNA, trans-activating CRISPR RNA, 55 bp DNA duplex non-target strand
Protein: CRISPR-associated endonuclease Cas9

Experimental Information Details

Resolution: 21Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN2, SPARX
Microscope: FEI TECNAI 20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)