EMDB EMD-5858

Single particle reconstruction
19Å resolution

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Map released:
Last modified:
Overview of EMD-5858
Source organism: Streptococcus pyogenes [1314]
Related EM entry by publication: EMD-5859, EMD-5860
Primary publication:
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
SCIENCE 343 1247997 (2014)
PMID: 24505130

Function and Biology Details

Sample name: apo-Cas9
Protein: CRISPR-associated endonuclease Cas9

Experimental Information Details

Resolution: 19Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN2, SPARX
Microscope: FEI TECNAI 20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)