EMDB EMD-5850

Subtomogram averaging
71Å resolution

Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP6 N-terminus tag)

Map released:
Last modified:
Overview of EMD-5850
Source organism: Chlamydomonas reinhardtii [3055]
Primary publication:
Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity
Oda T, Yanagisawa H, Yagi T, Kikkawa M
J.CELL BIOL. 204 807-819 (2014)
PMID: 24590175

Function and Biology Details

Sample name: Chlamydomonas axoneme, RSP6 BCCP N-terminus tag
Cellular component: Axoneme

Experimental Information Details

Resolution: 71Å
Resolution method: FSC 0.5, gold-standard
Applied symmetry: C1
Reconstruction software: PEET, RUBY-HELIX
Microscope: JEOL 3100FFC
Detector: TVIPS TEMCAM-F416 (4k x 4k)