EMD-5845

Subtomogram averaging
68.0 Å
EMD-5845
3D View Gallery
Deposition: 06/01/2014
Map released: 19/02/2014
Last modified: 07/05/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5845
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (Wild type)

EMD-5845

Subtomogram averaging
68.0 Å
EMD-5845
3D View Gallery
Deposition: 06/01/2014
Map released: 19/02/2014
Last modified: 07/05/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Filament
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 7.2
Details: 30 mM Hepes-NaOH pH 7.2, 5 mM MgCl2, 1 mM dithiothreitol, 1 mM EGTA, and 50 mM NaCl
Grid
Details: 300 mesh copper grid with home-made holey carbon support
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 93 K
Instrument: LEICA EM GP
Method: Blot for 5 seconds before plunging
Microscopy [1]
Microscope: JEOL 3100FFC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.2 mm
Nominal magnification: 15000.0
Calibrated magnification: 25700.0
Specimen holder model: GATAN LIQUID NITROGEN
Temperature
Minimum: 90 K
Maximum: 95 K
Specialist optics
Energy filter
Name: JEOL Omega
Lower energy threshold: 0.0 eV
Upper energy threshold: 23.0 eV
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Average electron dose per image: 90 e/Å2
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
-60.5 ° 60.5 ° - - - - -
Image processing [1]
Final reconstruction
Resolution: 68.0 Å ( BY AUTHOR)
Resolution method: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
PEET, RUBY-HELIX - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Averaged tomogram of axonemal doublet microtubules (Wild type)
Details: ::::EMDATABANK.org::::EMD-5845::::
⬡ Geometry
X Y Z
Dimensions 240 240 240
Origin 0 0 0
Spacing 240 240 240
Voxel size 6.07 Å 6.07 Å 6.07 Å
Contour list
Primary Level Source
True 34437.0 AUTHOR