EMDB EMD-5801

Single particle reconstruction
9.7Å resolution

Structure of the mammalian ribosomal pre-termination complex associated with eRF1-eRF3-GDPNP

Map released:
Last modified:
Overview of EMD-5801
Source organisms:
Replaces entries: EMD-5518
Fitted atomic model: 3j5y
Primary publication:
Structure of the mammalian ribosomal pre-termination complex associated with eRF1*eRF3*GDPNP.
des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J
NUCLEIC ACIDS RES. 42 3409-3418 (2014)
PMID: 24335085

Function and Biology Details

Sample name: eRF1-eRF3-GDPNP-bound mammalian ribosomal pre-termination complex
Nucleic acids: Transfer-messenger RNA Leu, messenger RNA
Proteins: eukaryotic release factor 1, eukaryotic release factor 3
Ribosome: 80S ribosome

Experimental Information Details

Resolution: 9.7Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: TVIPS TEMCAM-F415 (4k x 4k)