EMDB EMD-5799

Single particle reconstruction
7.6Å resolution

Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State

Map released:
Last modified:
Overview of EMD-5799
Source organisms:
Fitted atomic model: 4v7c
Related in-frame EM entries: EMD-5796, EMD-5797, EMD-5798, EMD-5800
Related EM entries by publication: EMD-5797, EMD-5796, EMD-5800, EMD-5798
Primary publication:
Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State
Brilot AF, Korostelev AA, Ermolenko DN, Grigorieff N
PROC.NAT.ACAD.SCI.USA 110 20994-20999 (2013)
PMID: 24324137

Function and Biology Details

Sample name: Pre-translocation ribosome with bound A/P, P/E hybrid state tRNA
Ligand: Viomycin
Nucleic acids: Transfer RNA, Messenger RNA
Ribosome: 70S ribosome

Experimental Information Details

Resolution: 7.6Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN2, IMAGIC, FREALIGN, RSAMPLE, CTFFIND3
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON I (4k x 4k)