EMDB EMD-5797

Single particle reconstruction
6.2Å resolution

Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State

Map released:
Last modified:
Overview of EMD-5797
Source organisms:
Related in-frame EM entries: EMD-5796, EMD-5798, EMD-5799, EMD-5800
Related EM entries by publication: EMD-5796, EMD-5800, EMD-5799, EMD-5798
Primary publication:
Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State
Brilot AF, Korostelev AA, Ermolenko DN, Grigorieff N
PROC.NAT.ACAD.SCI.USA 110 20994-20999 (2013)
PMID: 24324137

Function and Biology Details

Sample name: Post-translocation ribosome bound to EF-G with P, E sites occupied by tRNA
Ligands: Fusidic Acid, Viomycin
Nucleic acids: Transfer RNA, Messenger RNA
Protein: Elongation Factor G
Ribosome: 70S ribosome

Experimental Information Details

Resolution: 6.2Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN2, IMAGIC, FREALIGN, RSAMPLE, CTFFIND3
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON I (4k x 4k)