EMDB EMD-5723

Single particle reconstruction
22Å resolution

Negative stain Electron Microscopy of Bg505 SOSIP.664 in complex with sCD4 and 17b

Map released:
Overview of EMD-5723
Source organisms:
Related EM entry by publication: EMD-2427
Primary publication:
A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies.
Sanders RW, Derking R, Cupo A, Julien JP, Yasmeen A, de Val N, Kim HJ, Blattner C, de la Pena AT, Korzun J, Golabek M, de Los Reyes K, Ketas TJ, van Gils MJ, King CR, Wilson IA, Ward AB, Klasse PJ, Moore JP
PLOS PATHOG. 9 e1003618-e1003618 (2013)
PMID: 24068931

Function and Biology Details

Sample name: HIV spike protein 664G construct in complex with sCD4 and Fab fragment of 17b monoclonal antibody
Proteins: HIV spike protein BG505 SOSIP.664, sCD4, 17b Fab

Experimental Information Details

Resolution: 22Å
Resolution method: FSC 0.5
Applied symmetry: C3
Reconstruction software: EMAN2, EMAN1
Microscope: FEI TECNAI F20
Detector: TVIPS TEMCAM-F416 (4k x 4k)