EMDB EMD-5719

Single particle reconstruction
26Å resolution

Electron microscopy of the negatively-stained Cmr complex from Thermus thermophilus HB8.

Map released:
Overview of EMD-5719
Source organism: Thermus thermophilus [274]
Related EM entry by publication: EMD-2418
Primary publication:
Structure and Activity of the RNA-Targeting Type III-B CRISPR-Cas Complex of Thermus thermophilus.
Staals RH, Agari Y, Maki-Yonekura S, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJ, Yonekura K, van der Oost J, Shinkai A
MOLECULAR CELL 52 135-145 (2013)
PMID: 24119403

Function and Biology Details

Sample name: Cmr complex from Thermus thermophilus
Nucleic acid: crRNA
Proteins: Cmr2, Cmr3, Cmr1, Cmr4, Cmr5, Cmr6

Experimental Information Details

Resolution: 26Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN
Microscope: JEOL 2100