EMDB EMD-5693

Single particle reconstruction
10.1Å resolution

Structure of the SecY protein translocation channel in action

Map released:
Last modified:
Overview of EMD-5693
Source organism: Escherichia coli [562]
Fitted atomic model: 3j46
Related EM entry by publication: EMD-5691, EMD-5692
Primary publication:
Structure of the SecY channel during initiation of protein translocation.
Park E, Menetret JF, Gumbart JC, Ludtke SJ, Li W, Whynot A, Rapoport TA, Akey CW
NATURE 506 102-106 (2014)
PMID: 24153188

Function and Biology Details

Sample name: ribosome-nascent chain-SecYEG complex
Nucleic acid: transfer RNA
Proteins: preprotein translocase, S1P
Ribosome: membrane-bound 70S ribosome

Experimental Information Details

Resolution: 10.1Å
Resolution method: FSC at 0.5 cut-off for a comparison between the full experimental 3D density map and a calculated map of the docked E. coli ribosome model (this map was calculated to 7 Angstrom resolution with EMAN).
Applied symmetry: C1
Reconstruction software: EMAN2
Microscope: FEI TECNAI F20
Detector: GENERIC TVIPS (4k x 4k)