EMDB EMD-5692

Single particle reconstruction
9.5Å resolution

Structure of the SecY protein translocation channel in action

Map released:
Last modified:
Overview of EMD-5692
Source organism: Escherichia coli [562]
Fitted atomic model: 3j45
Related EM entry by publication: EMD-5691, EMD-5693
Primary publication:
Structure of the SecY channel during initiation of protein translocation.
Park E, Menetret JF, Gumbart JC, Ludtke SJ, Li W, Whynot A, Rapoport TA, Akey CW
NATURE 506 102-106 (2014)
PMID: 24153188

Function and Biology Details

Sample name: E. coli 70S ribosome with recombinant E. coli SecYEG
Protein: SecYEG
Ribosome: non-translating 70S ribosome

Experimental Information Details

Resolution: 9.5Å
Resolution method: Comparison of 3D map with calculated map of docked ribosomal components, with the second map made with EMAN at 7 Angstrom resolution.
Applied symmetry: C1
Reconstruction software: EMAN1
Microscope: FEI TECNAI 20
Detector: KODAK SO-163 FILM