EMD-5691

Single-particle
9.0 Å
EMD-5691
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Deposition: 14/06/2013
Map released: 23/10/2013
Last modified: 23/07/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5691
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4v4n summary report (pdf.gz) Map and 4v4n FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of the SecY protein translocation channel in action

EMD-5691

Single-particle
9.0 Å
EMD-5691
3D View Gallery
Deposition: 14/06/2013
Map released: 23/10/2013
Last modified: 23/07/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Methanococcus jannaschii 70S ribosome-SecYEbeta complex
Sample [1]
Name: Methanococcus jannaschii 70S ribosome-SecYEbeta complex
Number of unique components: 3
Oligomeric state: one 70S, one E-site tRNA, one SecYEbeta channel
Details: The sample was reconstituted from ribosomal subunits purified from M. jannaschii and from recombinant SecYEbeta.
Molecular weight
Experimental Theoretical Method
- 2 MDa -
Complex [1]
Name: non-translating 70S ribosome
Ribosome details: Ribosome-prokaryote: ALL
Natural source [1]
Organism: Methanocaldococcus jannaschii
Cellular location: cytoplasm
Recombinant Expression [1]
Organism Strain Cell Plasmid
- - - -
Molecular weight
Experimental Theoretical Method
- 2 MDa -
Protein [1]
Recombinant expression
Name: SecYEbeta (SecY channel)
Number of copies: 1
Oligomeric state: heterotrimer
Details: SecY: Q60175.2, SecE: Q57817.1, Secbeta: P60460.1
Natural source
Organism: Methanocaldococcus jannaschii
Cellular location: inner membrane
Molecular weight
Experimental Theoretical Method
- 61 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli C43(DE3) - pBAD22
RNA [1]
TRANSFER DOUBLE HELIX
Name: transfer RNA (tRNA)
Details: Co-purified with the ribosomal subunits
Natural source
Organism: Methanocaldococcus jannaschii
Molecular weight
Experimental Theoretical Method
- 25 kDa -