Single particle reconstruction
9.0Å resolution

Structure of the SecY protein translocation channel in action

Map released:
Last modified:
Overview of EMD-5691
Source organism: Methanocaldococcus jannaschii [2190]
Fitted atomic model: 4v4n
Related EM entry by publication: EMD-5692, EMD-5693
Primary publication:
Structure of the SecY channel during initiation of protein translocation.
Park E, Menetret JF, Gumbart JC, Ludtke SJ, Li W, Whynot A, Rapoport TA, Akey CW
NATURE 506 102-106 (2014)
PMID: 24153188

Function and Biology Details

Sample name: Methanococcus jannaschii 70S ribosome-SecYEbeta complex
Nucleic acid: transfer RNA
Protein: SecYEbeta
Ribosome: non-translating 70S ribosome

Experimental Information Details

Resolution: 9.0Å
Resolution method: FSC at 0.5 cut-off using a comparison between experimental map and a map of the docked ribosomal models calculated at 7 Angstrom resolution with EMAN
Applied symmetry: C1
Reconstruction software: EMAN1, EMAN2
Microscope: FEI TECNAI F20
Detector: KODAK SO-163 FILM