EMD-5659 Experiments and Validation

Cryo-electron microscopy of herpesvirus simplex type 1 C-capsid

Single particle reconstruction
Overview of EMD-5659
Sample name: Herpesvirus simplex type 1 C-capsid
Organism: Human herpesvirus 1

Map parameters

Recommended contour level: 5,000.00 (author)
Number of grid points: 723 × 723 × 723
Voxel size: 2.12 × 2.12 × 2.12 Å
Minimum density: -19,502.00
Maximum density: 32,443.00
Average density: 2,867.701
Standard deviation: 6,776.733

Sample information

Sample name: Herpesvirus simplex type 1 C-capsid
Virus: Human herpesvirus 1

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM(v3.13)
Number of particles: 3687
Reconstruction protocol: Fourier methods
CTF correction: Each micrograph

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 29000.0
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 48
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 6.3499999999999996

Specimen

Preparation
Specimen state: particle
Buffer: TNE: 500mM NaCl, 10mM Tris, 1mM EDTA
Specimen support: Quantifoil R2/1, 200 mesh copper, glow discharged for 10-15 secs
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE-PROPANE MIXTURE
Humidity: 95
Protocol: Blot for 6 seconds.