EMD-5656 Experiments and Validation

Pseudorabies GS-2168 B-capsid from cryo-electron microscopy

Single particle reconstruction
Overview of EMD-5656
Sample name: Pseudorabies virus GS-2168 UL25 - null mutant grown on PK15 cells
Organism: Suid herpesvirus 1

Map parameters

Minimum density: -21,154.00
Maximum density: 32,443.00
Average density: 1,054.094
Standard deviation: 5,627.495
Recommended contour level: 2,500.00 (author)

Sample information

Sample name: Pseudorabies virus GS-2168 UL25 - null mutant grown on PK15 cells
Virus: Suid herpesvirus 1

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM(v3.07)
Number of particles: 5870
Reconstruction protocol: Fourier methods
CTF correction: Each micrograph

Imaging

Session
Microscope model: FEI TECNAI 20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 29000.0
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 41
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 6.3499999999999996

Specimen

Preparation
Specimen state: particle
Buffer: TNE: 500mM NaCl, 10mM Tris, 1mM EDTA
Specimen support: Quantifoil R2/1, 200 mesh copper, glow discharged for 10-15 secs
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE-PROPANE MIXTURE
Humidity: 95
Protocol: Blot for 6 seconds.