EMD-5652
Pseudorabies virus B-capsid from cryo-electron microscopy
EMD-5652
Single-particle15.9 Å
Deposition: 01/05/2013
Map released: 10/07/2013
Last modified: 04/09/2013
Buffer
pH: 7.5
Details: TNE: 500mM NaCl, 10mM Tris, 1mM EDTA
Details: TNE: 500mM NaCl, 10mM Tris, 1mM EDTA
Grid
Details: Quantifoil R2/1, 200 mesh copper, glow discharged for 10-15 secs
Vitrification
Cryogen name: ETHANE-PROPANE MIXTURE
Chamber humidity: 95%
Chamber temperature: 82 K
Instrument: FEI VITROBOT MARK III
Method: Blot for 6 seconds.
Chamber humidity: 95%
Chamber temperature: 82 K
Instrument: FEI VITROBOT MARK III
Method: Blot for 6 seconds.
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal magnification: 39000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Polara stage
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal magnification: 39000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Polara stage
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 47
Bits per pixel: 8.0
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 47
Bits per pixel: 8.0
Details: Particles were selected with x3dpreprocess.
Final
reconstruction
Resolution: 15.9
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1111
Algorithm: OTHER
Details:
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1111
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group:
I
Software
[1]
Name | Version | Details |
---|---|---|
AUTO3DEM | - | - |
CTF correction
Details:Each micrograph
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Reconstruction of pseudorabies virus B-capsid
Details: ::::EMDATABANK.org::::EMD-5652::::
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Reconstruction of pseudorabies virus B-capsid
Details: ::::EMDATABANK.org::::EMD-5652::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 823 | 823 | 823 |
Origin | 0 | 0 | 0 |
Spacing | 823 | 823 | 823 |
Voxel size | 1.63 Å | 1.63 Å | 1.63 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 4000.0 | AUTHOR |