EMD-5632 Experiments and Validation

Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgG mutant

Subtomogram averaging
Overview of EMD-5632
Sample name: In situ flagellar motor from B. burgdorferi FlgG mutant cells
Organism: Borrelia burgdorferi
Related in-frame EM entries: EMD-5627, EMD-5628, EMD-5629, EMD-5630, EMD-5631, EMD-5633
Related EM entries by publication: EMD-5629, EMD-5628, EMD-5627, EMD-5630, EMD-5631, EMD-5633

Map parameters

Minimum density: -15.03
Maximum density: 13.874
Average density: 0.00
Standard deviation: 1.00
Recommended contour level: 2.50 (author)

Sample information

Sample name: In situ flagellar motor from B. burgdorferi FlgG mutant cells
Cellular component: flagellar motor

Validation information

Experimental information


Image processing

Applied symmetry: C1
Software: IMOD, Raptor, Protomo
Number of subtomograms averaged: 999
Number of class averages: 4
Reconstruction protocol: subvolume averaging


Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Nominal CS (mm): 2.00
Nominal magnification: 31000.0
Defocus max (nm): 8,000.00
Defocus min (nm): 6,000.00
Tilt max (degrees): 65.00
Tilt min (degrees): -65.00
Holder model: OTHER
Number of images: 8265


Specimen state: cell
Buffer: BSK-II liquid medium supplemented with 6% rabbit serum
Specimen support: freshly glow-discharged holey carbon grids
Cryogen: ETHANE
Protocol: Bacterial culture was deposited onto freshly glow-discharged, holey carbon grids for 1 min. Grids were blotted with filter paper and then rapidly frozen in liquid ethane, using a homemade gravity-driven plunger apparatus.