Sample information
Sample name: In situ flagellar motor from B. burgdorferi FlgB mutant cells
Cellular component:
flagellar motor
Validation information
Experimental information
Image processing
Applied
symmetry: C1
Software: IMOD, Raptor, Protomo
Number of
subtomograms averaged:
999
Number of
class averages: 4
Reconstruction
protocol: subvolume averaging
Imaging
Session
Microscope
model: FEI POLARA 300
Electron
source: FIELD EMISSION GUN
Electron
dose (e/A**2): 100.0
Nominal
CS (mm):
2.00
Nominal
magnification: 31000.0
Defocus
max (nm):
8,000.00
Defocus
min (nm):
6,000.00
Tilt max
(degrees):
65.00
Tilt min
(degrees):
-65.00
Holder
model: OTHER
Acquisition
Number
of images: 11832
Specimen
Preparation
Specimen
state: cell
Buffer:
BSK-II liquid medium supplemented with 6% rabbit serum
Specimen
support: freshly glow-discharged holey carbon grids
Vitrification
Apparatus:
HOMEMADE PLUNGER
Cryogen:
ETHANE
Protocol:
Bacterial culture was deposited onto freshly glow-discharged, holey carbon grids for 1 min. Grids were blotted with filter paper and then rapidly frozen in liquid ethane, using a homemade gravity-driven plunger apparatus.