EMD-5616 Experiments and Validation

Yeast 20S proteasome with C-terminal peptide of yeast Rpt1

Single particle reconstruction
Overview of EMD-5616
Sample name: Yeast 20S proteasome
Organism: Saccharomyces cerevisiae
Related EM entries by publication: EMD-5593, EMD-5611, EMD-5612, EMD-5613, EMD-5614, EMD-5615, EMD-5617

Map parameters

Recommended contour level: 0.80 (author)
Number of grid points: 200 × 200 × 200
Voxel size: 1.47 × 1.47 × 1.47 Å
Minimum density: -0.723
Maximum density: 2.077
Average density: -0.028
Standard deviation: 0.165

Sample information

Sample name: Yeast 20S proteasome
Protein: 20S proteasome

Validation information

Experimental information

 

Image processing

Applied symmetry: C2
Software: FREALIGN
Number of particles: 51135
CTF correction: Each Particle

Fitting

Initial atomic model: 1RYP
Fitting software: Chimera
Fitting protocol: rigid body
Refinement space: REAL

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 25.0
Nominal CS (mm): 2.00
Nominal magnification: 100000.0
Defocus max (nm): 2,100.00
Defocus min (nm): 1,200.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Details: Low-dose procedure

Specimen

Preparation
Specimen state: particle
Specimen support: Quantifoil grid
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE
Humidity: 100