Single particle reconstruction
26Å resolution

Substrate-specific structural rearrangements of human Dicer

Map released:
Last modified:
Overview of EMD-5604
Source organism: Homo sapiens [9606]
Related EM entries by publication: EMD-5605, EMD-5606, EMD-5601, EMD-5603, EMD-5602
Primary publication:
Substrate-specific structural rearrangements of human Dicer.
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
NAT.STRUCT.MOL.BIOL. 20 662-670 (2013)
PMID: 23624860

Function and Biology Details

Sample name: Human Dicer-PACT heterodimer in closed conformation
Proteins: Endoribonuclease Dicer, Interferon-inducible double stranded RNA-dependent protein kinase activator A

Experimental Information Details

Resolution: 26Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN2/SPARX, multi-model
Microscope: FEI TECNAI 12
Detector: GENERIC TVIPS (4k x 4k)