EMD-5592 Experiments and Validation

Electron cryo-microscopy of human 80S ribosome

Single particle reconstruction
Overview of EMD-5592
Sample name: Human 80S ribosome from PBMCs
Organism: Homo sapiens
Fitted atomic model: 4v6x
Related EM entry by publication: EMD-5591

Map parameters

Recommended contour level: 0.70 (author)
Number of grid points: 274 × 250 × 261
Voxel size: 1.24 × 1.24 × 1.24 Å
Minimum density: -0.773
Maximum density: 3.075
Average density: 0.081
Standard deviation: 0.258

Sample information

Sample name: Human 80S ribosome from PBMCs
Ribosome: 80S ribosome

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Spider
Number of particles: 343343
Reconstruction protocol: Projection matching
CTF correction: each subvolume

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 20.0
Nominal CS (mm): 2.70
Nominal magnification: 90000.0
Defocus max (nm): 3,500.00
Defocus min (nm): 800.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 30000

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Protocol: Before plunging, blot for 3 seconds using two layers of filter paper.