EMD-5591 Experiments and Validation

Electron cryo-microscopy of Drosophila melanogaster EF2- and Vig2-bound 80S ribosome

Single particle reconstruction
Overview of EMD-5591
Sample name: 80S D. melanogaster ribosome
Organism: Drosophila melanogaster
Fitted atomic model: 4v6w
Related EM entry by publication: EMD-5592

Map parameters

Recommended contour level: 0.49 (author)
Number of grid points: 368 × 368 × 368
Voxel size: 1.24 × 1.24 × 1.24 Å
Minimum density: -0.877
Maximum density: 1.943
Average density: 0.003
Standard deviation: 0.168

Sample information

Sample name: 80S D. melanogaster ribosome
Ribosome: 80S ribosome with E-tRNA, EF-2, and Vig2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Spider
Number of particles: 134500
Reconstruction protocol: Projection matching
CTF correction: each subvolume

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 20.0
Nominal CS (mm): 2.70
Nominal magnification: 90000.0
Defocus max (nm): 4,000.00
Defocus min (nm): 1,000.00
Holder model: GATAN LIQUID NITROGEN

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Protocol: Before plunging, blot for 3 seconds using two layers of filter paper.