Sample information
Sample name: Mature Sulfolobus Turreted Icosahedral Virus
Virus:
Sulfolobus turreted icosahedral virus
Validation information
Experimental information
Image processing
Applied
symmetry: I
Software: Frealign
Number of
particles: 8903
Reconstruction
protocol: Projection matching
CTF
correction: Each particle
Details: The final reconstruction was sharpened with a negative temperature factor of 400A2.
Fitting
Fitting 1
Initial
atomic model:
2BBD
Fitting
software: CNS
Refinement
space: RECIPROCAL
Details:
Positional (xyz) minimization and isotropic B factor refinement. Real space refinement was also carried out using Coot.
Fitting 2
Initial
atomic model:
4IL7
Fitting
software: CNS
Refinement
space: RECIPROCAL
Details:
Positional (xyz) minimization and isotropic B factor refinement. Real space refinement was also carried out using Coot. Only the N-terminal domain was refined using CNS (residues 3-134). The C-terminal domain (residues 135-222) was only rigid-body fit using Coot and an arbitrary B-factor value of 150 A2 was assigned to it.
Imaging
Session
Microscope
model: FEI TITAN KRIOS
Electron
source: FIELD EMISSION GUN
Electron
dose (e/A**2): 18.0
Nominal
CS (mm):
2.70
Defocus
max (nm):
2,500.00
Defocus
min (nm):
800.00
Tilt max
(degrees):
0.00
Tilt min
(degrees):
0.00
Holder
model: FEI TITAN KRIOS AUTOGRID HOLDER
Details:
Nanoprobe mode was used
Acquisition
Number
of images: 878
Specimen
Preparation
Specimen
state: particle
Buffer:
23 mM KH2PO4, 19 mM (NH4)2SO4, 1 mM MgSO4, 2 mM CaCl2
Specimen
support: glow-discharged C-flat holey carbon grids (CF-2/2-4C, Protochips)
Vitrification
Apparatus:
HOMEMADE PLUNGER
Cryogen:
ETHANE
Details:
Vitrification carried out at room temperature