EMD-5581 Experiments and Validation

Negatively Stained TEM structure of trypanosomatid core editing complex (L-complex)

Single particle reconstruction
Overview of EMD-5581
Sample name: L-Complex from L. major
Organism: Leishmania major

Map parameters

Minimum density: -2.8
Maximum density: 12.02
Average density: 0
Standard deviation: 0.91
Recommended contour level: 5.82 (author)

Sample information

Sample name: L-Complex from L. major
Protein: Core editing complex

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: EMAN
Number of particles: 12500
Number of class averages: 879
Reconstruction protocol: projection matching
CTF correction: phase flip

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 400.0
Nominal CS (mm): 2
Nominal magnification: 70000.0
Defocus max (nm): 2,100
Defocus min (nm): 1,600
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Number of images: 26
Sampling interval (μm): 15.0

Specimen

Preparation
Specimen state: particle
Buffer: 20 mM Tris, pH 7.6, 60 mM KCl, 10 mM MgCl2
Staining procedure: 2% uranyl acetate for 2 min
Specimen support: continuous carbon grid
Vitrification
Apparatus: NONE
Cryogen: NONE