EMD-5565 Experiments and Validation

CS-tubulin Kinesin-13 Microtubule complex

Helical reconstruction
Overview of EMD-5565
Sample name: kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Organisms: Drosophila melanogaster, Bos taurus
Fitted atomic model: 3j2u

Map parameters

Minimum density: -0.124
Maximum density: 0.159
Average density: 0.00
Standard deviation: 0.025
Recommended contour level: 0.015 (emdb)

Sample information

Sample name: kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Proteins: KLP10A, Tubulin alpha-1A chain, Tubulin beta-2B chain

Validation information

Experimental information

 

Image processing

Software: Spider, IHRSR, CTFFIND3, Custom
CTF correction: Each particle

Imaging

Session 1
Microscope model: JEOL 3200FSC
Electron source: FIELD EMISSION GUN
Holder model: JEOL
Session 2
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: CREO EVERSMART SUPREME

Specimen

Preparation
Specimen state: helicalArray
Aggregation parameters (helical parameters)
Handedness: RIGHT HANDED
Phi increment (degrees): 168.08743000000001
Azimuthal (Z) increment (Å): 5.55267
Vitrification
Apparatus: FEI VITROBOT MARK I
Cryogen: ETHANE