EMD-5528 Experiments and Validation

Cryo-EM structure of the contracted bacteriophage T4 tail containing the collar and whiskers made of fibritin molecules.

Single particle reconstruction
Overview of EMD-5528
Sample name: bacteriophage T4
Organism: Enterobacteria phage T4
Fitted atomic model: 3j2o

Map parameters

Recommended contour level: 2.00 (author)
Number of grid points: 250 × 250 × 250
Voxel size: 3.57 × 3.57 × 3.57 Å
Minimum density: -7.675
Maximum density: 11.692
Average density: 0.00
Standard deviation: 1.00

Sample information

Sample name: bacteriophage T4
Virus: Enterobacteria phage T4

Validation information

Experimental information

 

Image processing

Applied symmetry: C6
Software: EMAN
Number of particles: 2727
Reconstruction protocol: projection matching
CTF correction: phase flipping
Details: The reconstruction of the contracted T4 tail which lacked the collar and whiskers (EMDB accession code 1086; Leiman et al., 2004, Cell 118, 419-429) was used as the initial model.

Imaging

Session
Microscope model: FEI/PHILIPS CM200FEG
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 16.0
Nominal CS (mm): 2.00
Nominal magnification: 38000.0
Defocus max (nm): 3,000.00
Defocus min (nm): 1,500.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 97
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: 50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 8 mM MgCl2
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE