Single particle reconstruction
17.5Å resolution

Reconstruction of the deltaGCC mutant HCV IRES bound to rabbit 40S ribosomal subunit

Map released:
Overview of EMD-5527
Source organisms:
Primary publication:
HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation.
Filbin ME, Vollmar BS, Shi D, Gonen T, Kieft JS
NAT.STRUCT.MOL.BIOL. 20 150-158 (2013)
PMID: 23262488

Function and Biology Details

Sample name: mutant HCV IRES bound to 40S
Nucleic acid: hepatitis C virus internal ribosome entry site
Ribosome: Rabbit 40S ribosomal subunit

Experimental Information Details

Resolution: 17.5Å
Resolution method: RMEASURE
Applied symmetry: C1
Reconstruction software: FREALIGN
Microscope: FEI TECNAI F20
Detector: GENERIC TVIPS (4k x 4k)