EMD-5497 Experiments and Validation

Structure of ABCA4, the photoreceptor-specific ATP-binding cassette transporter

Single particle reconstruction
Overview of EMD-5497
Sample name: ABCA4 purified from bovine rod outer segments
Organism: Bos taurus
Related EM entry by publication: EMD-5498

Map parameters

Recommended contour level: 0.03 (author)
Number of grid points: 144 × 144 × 144
Voxel size: 2.13 × 2.13 × 2.13 Å
Minimum density: -0.064
Maximum density: 0.083
Average density: -0.001
Standard deviation: 0.009

Sample information

Sample name: ABCA4 purified from bovine rod outer segments
Protein: ABCA4

Validation information

Experimental information


Image processing

Applied symmetry: C1
Software: Spider
Number of particles: 25188
Reconstruction protocol: reference-based projection alignment
CTF correction: Each defocus group


Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal magnification: 50000.0
Defocus max (nm): 1,200.00
Defocus min (nm): 200.00
Tilt max (degrees): 45.00
Tilt min (degrees): 0.00
Holder model: OTHER
Number of images: 200


Specimen state: particle
Specimen concentration (mg/mL): 0.10
Buffer: 20 mM BTP, pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT
Staining procedure: Grids with adsorbed protein were stained with 1% w/v uranyl acetate for 10 seconds
Specimen support: Glow discharged Cu/Pd 400 mesh grids with thin carbon support.
Apparatus: NONE
Cryogen: NONE