EMD-5494 Experiments and Validation

Cryo-EM structure of short shafted adenovirus type 5 complexed with factor X

Single particle reconstruction
Overview of EMD-5494
Sample name: Complex of Ad5-short-fiber with factor X
Organism: Human adenovirus 5

Map parameters

Minimum density: 0.823
Maximum density: 1.273
Average density: 0.987
Standard deviation: 0.036
Recommended contour level: 0.995 (author)

Sample information

Sample name: Complex of Ad5-short-fiber with factor X
Virus: Human adenovirus 5

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: Frealign
Number of particles: 520
Reconstruction protocol: Fourier methods
CTF correction: Each particle

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 20.0
Nominal CS (mm): 2.26
Nominal magnification: 310000.0
Defocus max (nm): 2,100.00
Defocus min (nm): 500.00
Holder model: OTHER
Details: Low dose
Acquisition
Number of images: 1101
Scanner model: OTHER
Sampling interval (μm): 15.0

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.60
Buffer: 50mM Tris, 150mM NaCl, 2mM CaCl2, 2mM MgCl2
Specimen support: Quantifoil R2/4 holey carbon grids, glow discharged
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE
Humidity: 30
Protocol: Blot for 6 seconds before plunging