EMDB EMD-5451

Single particle reconstruction
17Å resolution

Bacteriophage phi29 prohead particle with a single insertion mutation (U92) in the pRNA

Map released:
Overview of EMD-5451
Source organism: Bacillus phage phi29 [10756]
Related EM entry by publication: EMD-2162
Primary publication:
A three-helix junction is the interface between two functional domains of prohead RNA in 29 DNA packaging.
Zhao W, Saha M, Ke A, Morais MC, Jardine PJ, Grimes S
J.VIROL. 86 11625-11632 (2012)
PMID: 22896620

Function and Biology Details

Sample name: Bacteriophage phi29 prohead particle with a double insertion mutation (U92,U93) in the pRNA
Virus: Bacillus phage phi29

Experimental Information Details

Resolution: 17Å
Resolution method: FSC 0.5
Applied symmetry: C5
Reconstruction software: EMAN
Microscope: JEOL 2200FS
Detector: GATAN ULTRASCAN 4000 (4k x 4k)