EMD-5449 Experiments and Validation

Dynamics in Cryo EM Reconstructions Visualized with Maximum-Likelihood Derived Variance Maps

Single particle reconstruction
Overview of EMD-5449
Sample name: Nudaurelia capensis omega virus (NwV) capsid at 3min time point following maturation initiation
Organism: Nudaurelia capensis omega virus
Related EM entries by publication: EMD-5474, EMD-5472, EMD-5473, EMD-5470, EMD-5471, EMD-5469, EMD-5468

Map parameters

Minimum density: -0.001
Maximum density: 0.001
Average density: 0.00
Standard deviation: 0.00
Recommended contour level: 0.00 (author)

Sample information

Sample name: Nudaurelia capensis omega virus (NwV) capsid at 3min time point following maturation initiation
Virus: Nudaurelia capensis omega virus

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Number of particles: 1200
Reconstruction protocol: Maximum Likelihood
CTF correction: Each image

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Holder model: GATAN LIQUID NITROGEN
Acquisition
Details: Data were collected using the Leginon automated electron microscopy package and data processing was performed using the Appion pipeline.

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK I
Cryogen: ETHANE