EMD-5444 Experiments and Validation

MDA5-dsRNA filament

Helical reconstruction
Overview of EMD-5444
Sample name: MDA5-dsRNA
Organism: Mus musculus

Map parameters

Recommended contour level: 0.175 (emdb)
Number of grid points: 100 × 100 × 100
Voxel size: 4.16 × 4.16 × 4.16 Å
Minimum density: -0.56
Maximum density: 0.995
Average density: 0.00
Standard deviation: 0.081

Sample information

Sample name: MDA5-dsRNA
Protein: MDA5

Validation information

Experimental information

 

Image processing

Software: Spider
Reconstruction protocol: IHRSR

Imaging

Session
Microscope model: FEI TECNAI 12
Electron source: TUNGSTEN HAIRPIN
Holder model: OTHER
Acquisition
Scanner model: NIKON COOLSCAN
Sampling interval (μm): 4.1600000000000001

Specimen

Preparation
Specimen state: filament
Aggregation parameters (helical parameters)
Handedness: RIGHT HANDED
Phi increment (degrees): 82.700000000000003
Azimuthal (Z) increment (Å): 43.600000000000001
Vitrification
Apparatus: NONE
Cryogen: NONE