EMD-5412 Experiments and Validation

CryoEM icosahedral reconstruction of AHSV7

Single particle reconstruction
Overview of EMD-5412
Sample name: AHSV7 truncated VP2 filled
Organism: African horse sickness virus 7
Related EM entry by publication: EMD-2075, EMD-2076

Map parameters

Recommended contour level: 1.00 (author)
Number of grid points: 513 × 513 × 513
Voxel size: 1.80 × 1.80 × 1.80 Å
Minimum density: -26,388.00
Maximum density: 32,443.00
Average density: 3,797.498
Standard deviation: 8,639.898

Sample information

Sample name: AHSV7 truncated VP2 filled
Virus: African horse sickness virus 7

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Number of particles: 2798
Reconstruction protocol: polar Fourier transform
CTF correction: each micrograph

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 18.0
Nominal CS (mm): 2.00
Nominal magnification: 83000.0
Defocus max (nm): 5,000.00
Defocus min (nm): 800.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 507

Specimen

Preparation
Specimen state: particle
Buffer: 2mM Tris-HCl
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE
Humidity: 100