EMD-5411 Experiments and Validation

The Human Nuclear Pore Complex as Revealed by Cryo-Electron Tomography

Subtomogram averaging
Overview of EMD-5411
Sample name: Asymmetric unit of the human nuclear pore complex
Organism: Homo sapiens

Map parameters

Recommended contour level: 0.115 (author)
Number of grid points: 128 × 128 × 128
Voxel size: 8.20 × 8.20 × 8.20 Å
Minimum density: -0.378
Maximum density: 0.485
Average density: 0.005
Standard deviation: 0.074

Sample information

Sample name: Asymmetric unit of the human nuclear pore complex
Cellular component: nuclear pore complex

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: TOM_toolbox_software_package
Number of subtomograms averaged: 1696
Reconstruction protocol: missing wedge weighted correlation averaging

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Energy filter: GIF2002
Nominal magnification: 18000.0
Defocus max (nm): 1,200.00
Defocus min (nm): 1,200.00
Tilt max (degrees): 60.00
Tilt min (degrees): -60.00
Holder model: GATAN LIQUID NITROGEN

Specimen

Preparation
Specimen state: particle
Buffer: Dulbecco phosphate buffer saline
Specimen support: 200 mesh gold grids, silicon coated
Vitrification
Apparatus: OTHER
Cryogen: ETHANE
Protocol: Blot for 4 seconds before plunging