EMD-5387 Experiments and Validation

Structure of the Fab-Labeled 'Breathing' State of Native Poliovirus

Single particle reconstruction
Overview of EMD-5387
Sample name: Poliovirus 160S particle at 37 degrees C, Fab complex
Organism: Human poliovirus 1 Mahoney

Map parameters

Minimum density: -16.281
Maximum density: 48.292
Average density: 5.327
Standard deviation: 12.598
Recommended contour level: 5.30 (author)

Sample information

Sample name: Poliovirus 160S particle at 37 degrees C, Fab complex
Virus: Human poliovirus 1 Mahoney

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: EM3DR2
Number of particles: 137
Reconstruction protocol: Fourier Bessel
CTF correction: CTF and decay correct

Imaging

Session
Microscope model: FEI TECNAI F30
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 10.0
Nominal CS (mm): 2.00
Nominal magnification: 39000.0
Defocus max (nm): 3,037.00
Defocus min (nm): 2,395.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 7
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: 20 mM Tris, 2 mM CaCl2
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE