EMD-5380 Experiments and Validation

Cryo-EM 3D reconstruction of empty Triatoma Virus (corresponding to a suspension of full TrV heated)

Single particle reconstruction
Overview of EMD-5380
Sample name: Empty TrV particles
Organism: Triatoma virus
Related EM entry by publication: EMD-2025, EMD-5379

Map parameters

Minimum density: -0.003
Maximum density: 0.004
Average density: 0.00
Standard deviation: 0.001
Recommended contour level: 0.001 (author)

Sample information

Sample name: Empty TrV particles
Virus: Triatoma virus

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: EM3DR2
Number of particles: 573

Imaging

Session
Microscope model: FEI/PHILIPS CM200T
Electron source: LAB6
Nominal CS (mm): 2.00
Nominal magnification: 38000.0
Defocus max (nm): 4,000.00
Defocus min (nm): 1,200.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 15
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: NMT (10 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl)
Specimen support: 400 mesh quantifoil grid
Vitrification
Apparatus: FEI VITROBOT
Cryogen: ETHANE
Details: Vitrification instrument: Vitrobot FEI