EMD-5342 Experiments and Validation

DNA-free p53 Tetramer Reconstruction

Single particle reconstruction
Overview of EMD-5342
Sample name: p53
Organism: Homo sapiens

Map parameters

Recommended contour level: 0.157 (author)
Number of grid points: 120 × 120 × 120
Voxel size: 2.20 × 2.20 × 2.20 Å
Minimum density: -0.039
Maximum density: 0.70
Average density: 0.008
Standard deviation: 0.051

Sample information

Sample name: p53
Protein: p53

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 8550
Number of class averages: 50

Imaging

Session
Microscope model: FEI TECNAI SPHERA
Electron source: LAB6
Holder model: PHILIPS ROTATION HOLDER
Acquisition
Number of images: 50

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.00
Buffer: 150mM NaCl
Staining procedure: 1% uranyl formate
Vitrification
Apparatus: NONE
Cryogen: NONE