Single particle reconstruction
25Å resolution

3D reconstruction of negatively stained PCSK9 in complex with a Fab

Map released:
Overview of EMD-5295
Source organism: Homo sapiens [9606]
Related EM entry by publication: EMD-5294
Primary publication:
Fabs enable single particle cryoEM studies of small proteins.
Wu S, Avila-Sakar A, Kim J, Booth DS, Greenberg CH, Rossi A, Liao M, Li X, Alian A, Griner SL, Juge N, Yu Y, Mergel CM, Chaparro-Riggers J, Strop P, Tampe R, Edwards RH, Stroud RM, Craik CS, Cheng Y
STRUCTURE 20 582-592 (2012)
PMID: 22483106

Function and Biology Details

Sample name: human PCSK9 in complex with a Fab
Protein: PCSK9

Experimental Information Details

Resolution: 25Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: Frealign
Microscope: FEI TECNAI 20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)