Single particle reconstruction
9.6Å resolution

3D reconstruction of frozen hydrated HIV-1 integrase dimer in complex with two Fabs.

Map released:
Overview of EMD-5294
Source organism: Human immunodeficiency virus 1 [11676]
Related EM entry by publication: EMD-5295
Primary publication:
Fabs enable single particle cryoEM studies of small proteins.
Wu S, Avila-Sakar A, Kim J, Booth DS, Greenberg CH, Rossi A, Liao M, Li X, Alian A, Griner SL, Juge N, Yu Y, Mergel CM, Chaparro-Riggers J, Strop P, Tampe R, Edwards RH, Stroud RM, Craik CS, Cheng Y
STRUCTURE 20 582-592 (2012)
PMID: 22483106

Function and Biology Details

Sample name: HIV-1 integrase - Fab complex
Protein: integrase

Experimental Information Details

Resolution: 9.6Å
Resolution method: FSC 0.143
Applied symmetry: C2
Reconstruction software: Frealign
Microscope: FEI TECNAI F20
Detector: TVIPS TEMCAM-F816 (8k x 8k)