EMDB EMD-5127

Single particle reconstruction
19.8Å resolution

Three-dimensional EM structure of an intact activator-dependent transcription initiation complex

Map released:
Last modified:
Overview of EMD-5127
Source organisms:
Fitted atomic model: 3iyd
Primary publication:
Three-dimensional EM structure of an intact activator-dependent transcription initiation complex.
Hudson BP, Quispe J, Lara S, Kim Y, Berman HM, Arnold E, Ebright RH, Lawson CL
PROC.NAT.ACAD.SCI.USA 106 19830-19835 (2009)
PMID: 19903881

Function and Biology Details

Sample name: E. coli RNA polymerase holoenzyme (sigma70) and E. coli catabolite activator protein (CAP) bound to 98-mer DNA containing the lac promoter and engineered open transcription bubble
Nucleic acid: lac(ICAP)UP-UV5-bubble
Proteins: Catabolite Activator Protein, RNA polymerase holoenzyme (sigma70)

Experimental Information Details

Resolution: 19.8Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN, SPIDER
Microscope: FEI TECNAI F20
Detector: TVIPS TEMCAM-F415 (4k x 4k)