EMD-4767

Single-particle
4.0 Å
EMD-4767 Deposition: 02/04/2019
Map released: 12/06/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4767

Cryo-EM structure of NCP-6-4PP

EMD-4767

Single-particle
4.0 Å
EMD-4767 Deposition: 02/04/2019
Map released: 12/06/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Buffer
pH: 7.4
Buffer components [3]:
Name Formula Concentration ChEBI
Sodium chloride NaCl 50.0 mM
HEPES - 50.0 mM -
TCEP - 0.25 mM -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 85%
Chamber temperature: 277 K
Instrument: LEICA EM GP
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 0.0 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Number of grids: 1
Average exposure time: 6.0 s
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 98378
Software [1]
Name Version Details
RELION 2 -
Initial angle assignment
Type: OTHER
Software [1]
Name Version Details
RELION - -
Final angle assignment
Type: OTHER
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Dimensions 300 300 300
Origin 0 0 0
Spacing 300 300 300
Voxel size 0.86 Å 0.86 Å 0.86 Å
Contour list
Primary Level Source
True 3.5 AUTHOR