EMD-4767
Cryo-EM structure of NCP-6-4PP
EMD-4767
Single-particle4.0 Å
Deposition: 02/04/2019
Map released: 12/06/2019
Last modified: 18/12/2019
Concentration: 2
mg/mL
Buffer
pH: 7.4
Buffer components [3]:
Buffer components [3]:
Name | Formula | Concentration | ChEBI |
---|---|---|---|
Sodium chloride | NaCl | 50.0 mM | |
HEPES | - | 50.0 mM | - |
TCEP | - | 0.25 mM | - |
Vitrification
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 0.0 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 0.0 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Number of grids: 1
Average exposure time: 6.0 s
Average electron dose per image: 40.0 e/Å2
Detector mode: COUNTING
Number of grids: 1
Average exposure time: 6.0 s
Average electron dose per image: 40.0 e/Å2
Final
reconstruction
Resolution: 4.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 98378
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 98378
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 2 | - |
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
Gctf | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 300 | 300 | 300 |
Origin | 0 | 0 | 0 |
Spacing | 300 | 300 | 300 |
Voxel size | 0.86 Å | 0.86 Å | 0.86 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 3.5 | AUTHOR |