EMDB EMD-4397

Single particle reconstruction
3.7Å resolution

Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex

Map released:
Overview of EMD-4397
Source organisms:
Fitted atomic model: 6gfw
Related EM entry by publication: EMD-0001, EMD-0002
Primary publication:
Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation.
Glyde R, Ye F, Jovanovic M, Kotta-Loizou I, Buck M, Zhang X
Mol. Cell 70 1111-1120.e3 (2018)
PMID: 29932903

Function and Biology Details

Sample name: Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex
Nucleic acids: nifH promoter template DNA, NifH promoter non-template DNA, de novo synthesized RNA
Proteins: Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex, DNA-directed RNA polymerase, Promoter DNA, RNA polymerase sigma-54 factor, de novo DNA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma-54 factor

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)