EMDB EMD-4384

Single particle reconstruction
4.32Å resolution

CryoEM structure of respiratory complex I from Yarrowia lipolytica

Map released:
Overview of EMD-4384
Source organism: Yarrowia lipolytica [4952]
Fitted atomic model: 6gcs
Related EM entry by publication: EMD-4385
Primary publication:
Cryo-EM structure of respiratory complex I at work.
Parey K, Brandt U, Xie H, Mills DJ, Siegmund K, Vonck J, Kuehlbrandt W, Zickermann V
Elife 7 - (2018)
PMID: 30277212

Function and Biology Details

Sample name: Mitochondrial NADH:ubiquinone oxidoreductase
Ligands: IRON/SULFUR CLUSTER, FE2/S2 (INORGANIC) CLUSTER, FLAVIN MONONUCLEOTIDE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ZINC ION, S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate, CARDIOLIPIN, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
Proteins: Mitochondrial NADH:ubiquinone oxidoreductase, 75-KDA PROTEIN (NUAM), 51-KDA PROTEIN (NUBM), 49-KDA PROTEIN (NUCM), NIMM SUBUNIT, NUEM SUBUNIT, NUFM SUBUNIT, 30-KDA PROTEIN (NUGM), 24-KDA SUBUNIT (NUHM), TYKY SUBUNIT (NUIM), NUJM SUBUNIT, PSST SUBUNIT (NUKM), ND4L SUBUNIT (NULM), NUMM SUBUNIT, ACPM1 SUBUNIT, NB4M SUBUNIT, ACPM2 SUBUNIT, NI2M SUBUNIT, NESM SUBUNIT, NUPM SUBUNIT, NB6M SUBUNIT, NUXM SUBUNIT, NUYM SUBUNIT, NUZM SUBUNIT, NIAM SUBUNIT, NEBM SUBUNIT, NB2M SUBUNIT, NIDM SUBUNIT, NUUM SUBUNIT, NI8M SUBUNIT, NI9M SUBUNIT, N7BM SUBUNIT, UNKNOWN SUBUNIT, NB5M SUBUNIT, NUNM SUBUNIT, ND1 SUBUNIT (NU1M), ND2 SUBUNIT (NU2M), ND3 SUBUNIT (NU3M), ND4 SUBUNIT (NU4M), ND5 SUBUNIT (NU5M), ND6 SUBUNIT (NU6M), NB8M SUBUNIT, NIPM SUBUNIT

Experimental Information Details

Resolution: 4.32Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)